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Electron Spin Resonance Spectroscopy Studies on Reduction Process of Nitroxyl Radicals used in Molecular Imaging

Received: 6 February 2014     Published: 28 February 2014
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Abstract

Electron spin resonance (ESR) spectroscopy studies on the reduction process of nitroxyl radicals were carried out for 1mM concentration of 14N-labeled nitroxyl radicals in 1 mM concentration of ascorbic acid as a function of time. The half life time and decay rate were estimated for 1mM concentration of 14N labeled nitroxyl radicals in 1 mM concentration of ascorbic acid. From the results, the increase in half life time and decrease in decay rate were calculated for TEMPONE compared with TEMPO and TEMPOL radicals, which indicates the higher stability of TEMPONE radical. The observed radical scavenging activity is also higher for TEMPONE radical. The ESR spectrum was also recorded for 1mM concentration of 14N-labeled nitroxyl radicals in pure water and the ESR parameters, line width, hyperfine coupling constant, g-factor, signal intensity ratio and rotational correlation time were obtained. These results indicate that the TEMPONE radical has narrowest line width and fast tumbling motion compared with TEMPO and TEMPOL. Therefore, this study reveals that the TEMPONE radical can act as a good redox sensitive spin probe for molecular imaging.

Published in European Journal of Biophysics (Volume 2, Issue 1)
DOI 10.11648/j.ejb.20140201.11
Page(s) 1-6
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Electron Spin Resonance Spectroscopy, Nitroxyl Radicals, Ascorbic Acid, Half Life Time, Decay Rate

References
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  • APA Style

    M. Kumara Dhas, A. Jawahar, A. Milton Franklin Benial. (2014). Electron Spin Resonance Spectroscopy Studies on Reduction Process of Nitroxyl Radicals used in Molecular Imaging. European Journal of Biophysics, 2(1), 1-6. https://doi.org/10.11648/j.ejb.20140201.11

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    M. Kumara Dhas; A. Jawahar; A. Milton Franklin Benial. Electron Spin Resonance Spectroscopy Studies on Reduction Process of Nitroxyl Radicals used in Molecular Imaging. Eur. J. Biophys. 2014, 2(1), 1-6. doi: 10.11648/j.ejb.20140201.11

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    AMA Style

    M. Kumara Dhas, A. Jawahar, A. Milton Franklin Benial. Electron Spin Resonance Spectroscopy Studies on Reduction Process of Nitroxyl Radicals used in Molecular Imaging. Eur J Biophys. 2014;2(1):1-6. doi: 10.11648/j.ejb.20140201.11

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  • @article{10.11648/j.ejb.20140201.11,
      author = {M. Kumara Dhas and A. Jawahar and A. Milton Franklin Benial},
      title = {Electron Spin Resonance Spectroscopy Studies on Reduction Process of Nitroxyl Radicals used in Molecular Imaging},
      journal = {European Journal of Biophysics},
      volume = {2},
      number = {1},
      pages = {1-6},
      doi = {10.11648/j.ejb.20140201.11},
      url = {https://doi.org/10.11648/j.ejb.20140201.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20140201.11},
      abstract = {Electron spin resonance (ESR) spectroscopy  studies on the reduction process of nitroxyl radicals were carried out for 1mM concentration of 14N-labeled nitroxyl radicals in 1 mM concentration of ascorbic acid as a function of time. The half life time and decay rate were estimated for 1mM concentration of 14N labeled nitroxyl radicals in 1 mM concentration of ascorbic acid. From the results, the increase in half life time and decrease in decay rate were calculated for TEMPONE compared with TEMPO and TEMPOL radicals, which indicates the higher stability of TEMPONE radical. The observed radical scavenging activity is also higher for TEMPONE radical. The ESR spectrum was also recorded for 1mM concentration of 14N-labeled nitroxyl radicals in pure water and the ESR parameters, line width, hyperfine coupling constant, g-factor, signal intensity ratio and rotational correlation time were obtained. These results indicate that the TEMPONE radical has narrowest line width and fast tumbling motion compared with TEMPO and TEMPOL. Therefore, this study reveals that the TEMPONE radical can act as a good redox sensitive spin probe for molecular imaging.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Electron Spin Resonance Spectroscopy Studies on Reduction Process of Nitroxyl Radicals used in Molecular Imaging
    AU  - M. Kumara Dhas
    AU  - A. Jawahar
    AU  - A. Milton Franklin Benial
    Y1  - 2014/02/28
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ejb.20140201.11
    DO  - 10.11648/j.ejb.20140201.11
    T2  - European Journal of Biophysics
    JF  - European Journal of Biophysics
    JO  - European Journal of Biophysics
    SP  - 1
    EP  - 6
    PB  - Science Publishing Group
    SN  - 2329-1737
    UR  - https://doi.org/10.11648/j.ejb.20140201.11
    AB  - Electron spin resonance (ESR) spectroscopy  studies on the reduction process of nitroxyl radicals were carried out for 1mM concentration of 14N-labeled nitroxyl radicals in 1 mM concentration of ascorbic acid as a function of time. The half life time and decay rate were estimated for 1mM concentration of 14N labeled nitroxyl radicals in 1 mM concentration of ascorbic acid. From the results, the increase in half life time and decrease in decay rate were calculated for TEMPONE compared with TEMPO and TEMPOL radicals, which indicates the higher stability of TEMPONE radical. The observed radical scavenging activity is also higher for TEMPONE radical. The ESR spectrum was also recorded for 1mM concentration of 14N-labeled nitroxyl radicals in pure water and the ESR parameters, line width, hyperfine coupling constant, g-factor, signal intensity ratio and rotational correlation time were obtained. These results indicate that the TEMPONE radical has narrowest line width and fast tumbling motion compared with TEMPO and TEMPOL. Therefore, this study reveals that the TEMPONE radical can act as a good redox sensitive spin probe for molecular imaging.
    VL  - 2
    IS  - 1
    ER  - 

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Author Information
  • Department of Physics, NMSSVN College, Madurai, India

  • Department of Chemistry, NMSSVN College, Madurai, India

  • Department of Physics, NMSSVN College, Madurai, India

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